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Nitric Oxide (NO) Colorimetric Assay Kit

Nitric Oxide Assay Kit measures NO production by reducing nitrate to nitrite using the Griess method. The mechanism involves an azo coupling between diazonium species and naphthylethylenediamine.

Product Specifications

Field of Research

Pharmacology & Drug Discovery

Storage Conditions

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Notes

For research use only.

Applications Notes

Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite by using improved Griess method. The Griess assay’s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2- and naphthylethyl -enediamine, resulting in a colorimetric (540 nm) product proportional to the NO metabolite present.

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