Glutathione Oxidized (GSSG) Colorimetric Assay Kit
Reduced glutathione can react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the maximum light absorption at 412 nm wavelength. The original reduced glutathione in the sample is inhibited by 2-vinylpyridine, and then GSSG is reduced to GSH by Glutathione Reductase to determine the content of GSSG.
Product Specifications
Field of Research
Pharmacology & Drug Discovery
Storage Conditions
Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Notes
For research use only.
Applications Notes
Reduced glutathione can react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the maximum light absorption at 412 nm wavelength. The original reduced glutathione in the sample is inhibited by 2-vinylpyridine, and then GSSG is reduced to GSH by Glutathione Reductase to determine the content of GSSG.
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