Glutathione Peroxidase (GSH-Px) Activity Colorimetric Assay Kit
GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+. NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not.
Product Specifications
Field of Research
Pharmacology & Drug Discovery
Storage Conditions
Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Notes
For research use only.
Applications Notes
GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.
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