Free Cholesterol (FC) Colorimetric Assay Kit
The kit provides a method for detecting FC concentration in biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2.
Product Specifications
Product Name Alternative
FC; cholesterol
Field of Research
Metabolism Research
Storage Conditions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Notes
For research use only.
Applications Notes
The kit provides a simple method for detecting FC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Free Cholesterol (FC) present in the sample is proportional to the signal obtained.
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