Total Cholesterol (TC) Colorimetric Assay Kit
The kit provides a method for detecting TC concentration in biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA) .
Product Specifications
Product Name Alternative
TC; cholesterol
Field of Research
Metabolism Research
Storage Conditions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Notes
For research use only.
Applications Notes
The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA) . Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.
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