Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit

Phosphopyruvate carboxylase; Phosphoenolpyruvic carboxylase; PHOSPHOENOLPYRUVATE CARBOXYLASE; Phosphoenolpyruvic acid cocarboxylase; Phospho (enol) pyruvate Carboxylase corn; Carboxylase, phosphopyruvate (phosphate)

Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

The kit provides a simple method for detecting Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, plasma, bacterial. In the assay, Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate and CO2 to produce oxaloacetate and HPO42-, and malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+. The rate of NADH decrease at 340 nm can reflect Phosphoenolpyruvate carboxylase (PEPC) activity.