Superoxide Anion Colorimetric Assay Kit

Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells . In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2-, and NO2- reacts with Gris’ reagent. The mechanism of Gris’ analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N- (1-naphthyl) ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value.