Alpha-Ketoglutarate Dehydrogenase (Alpha-KGDH) Assay Kit (Colorimetric)

α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit provides a simple, sensitive, rapid and colorimetric α-KGDH activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi. The principle of detection is that α-KGDH can catalyze α-Ketoglutarate, NAD + and coenzyme A to produce succinyl-coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. α-KGDH activity could be calculated according to NADH-generating rates.