6-Phosphofructokinase (PFK) Activity Colorimetric Assay Kit
6-Phosphofructokinase (PFK) Activity Assay Kit provides a colorimetric PFK detection method. PFK catalyzes fructose 6-phosphate and ATP to generate fructose-1,6-diphosphate and ADP, followed by NADH oxidation.
Product Specifications
References & Citations
Yohei Matsunaga et al. The pseudokinase domain PK1 of UNC-89/obscurin is required for mitochondrial morphology and function in C. elegans J Muscle Res Cell Motil ., (2026)
Pubmed id
42251225
Storage Conditions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Notes
For research use only.
Applications Notes
6-Phosphofructokinase (PFK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
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