Pyruvate Decarboxylase (PDC) Colorimetric Assay Kit

The kit provides a simple method for detecting PDC activity in a variety of biological samples such as tissues, cell, serum, bacterial. The principle is that PDC catalyzes the decarboxylation of pyruvate to produce acetaldehyde, and alcohol dehydrogenase (ADH) is added to further catalyze the reduction of acetaldehyde by NADH to produce ethanol and NAD+. NADH has an absorption peak at 340 nm, but NAD+ does not. PDC activity is calculated by measuring the rate of decrease in light absorption at 340 nm.