EdU Cell Proliferation Image Kit (Orange Fluorescence)

Cell Proliferation EdU Image Kit (Orange Fluorescence) is a novel alternative to the BrdU assay. EdU (5-ethynyl-2 ́-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, is complete within 30 minutes.