Nuclear Protein Extraction Kit
Nuclear Protein Extraction Kit enables the stepwise preparation of cytoplasmic and nuclear extracts from mammalian cells or tissues. The process uses a hypotonic buffer for cell swelling and disruption, followed by a high salt buffer to release nuclear proteins.
Product Specifications
Storage Conditions
Stored at-20°C for 12 months, please store refer to protocols.
Notes
For research use only.
Applications Notes
Nuclear Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.
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