SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (V367F)

Expression Region

Arg 319 - Phe 541

Host

HEK293

Target

Spike RBD

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (V367F), His Tag (Cat. No. SPD-S52H4) with a linear range of 1-16 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

27.0 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

1mg*1

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

QHD43416.1 (V367F)

Preservative

Trehalose

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