Laemmli Sample Buffer 2X Solution

<strong>Laemmli Sample Buffer 2X</strong>_x000D_ _x000D_ <strong>Catalog number:</strong> B2010105_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 4 x 2 mL_x000D_ <strong>pH:</strong> 6.8_x000D_ <strong>Supplied as:</strong> Ready-to-use solution (mix with sample 1:1)_x000D_ <strong>Appearance:</strong> Blue Solution_x000D_ <strong>Applications:</strong> denaturing and loading protein samples during SDS-PAGE._x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> Laemmli Buffer, SDS-PAGE loading buffer, Protein Denaturing buffer_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Karlsson JO, Ostwald K, Kåbjörn C, Andersson M. A method for protein assay in_x000D_ Laemmli buffer. Anal Biochem. 1994 May 15;219(1):144-6.2: Sheen H. Quantitation of yeast total proteins in sodium dodecyl sulfate-_x000D_ polyacrylamide gel electrophoresis sample buffer for uniform loading. Anal_x000D_ Biochem. 2016 Apr 1;498:95-7.3: Amponsah A, Nayak B. Evaluation of the efficiency of three extraction_x000D_ conditions for the immunochemical detection of allergenic soy proteins in_x000D_ different food matrices. J Sci Food Agric. 2018 Apr;98(6):2378-2384.4: Cai X, Zheng Y, Speck NA. A Western Blotting Protocol for Small Numbers of_x000D_ Hematopoietic Stem Cells. J Vis Exp. 2018 Aug 22;(138):56855._x000D_ _x000D_ 5: Scully RR, Pressley TA, O’Neil RG. A site-directed antibody recognizes a_x000D_ component of the ouabain-binding domain of the alpha 1 subunit of rat_x000D_ Na+,K(+)-ATPase. Biochem Cell Biol. 1993 Nov-Dec;71(11-12):538-43._x000D_ _x000D_ 6: Guthrie CR, Murray LW, Kopchok GE, Rosenbaum D, White RA. Biochemical_x000D_ mechanisms of laser vascular tissue fusion. J Invest Surg. 1991;4(1):3-12._x000D_ _x000D_ 7: Rendić D, Wilson IB, Lubec G, Gutternigg M, Altmann F, Léonard R. Adaptation_x000D_ of the “in-gel release method” to N-glycome analysis of low-milligram amounts of_x000D_ material. Electrophoresis. 2007 Dec;28(23):4484-92._x000D_ _x000D_ 8: Wang X, Wang H, Wang D, Wang D, Han B, Tian W, Guo A. Thermal denaturation_x000D_ produced degenerative proteins and interfered with MS for proteins dissolved in_x000D_ lysis buffer in proteomic analysis. <a href="https://pubmed.ncbi.nlm.nih.gov/21298662/">Electrophoresis</a>. 2011 Feb;32(3-4):348-56._x000D_ _x000D_ <strong>Products Related to Laemmli Sample Buffer 2X Solution: </strong> <a>Biochemical Buffers and Solutions</a>