Goat anti-ZIC5 Antibody
Goat polyclonal antibody to ZIC5
Product Specifications
Product Name Alternative
Anti ZIC5 antibody, anti zinc finger protein of the cerebellum 5 antibody, anti zinc family member 5 protein antibody, anti Zic family member 5 (odd-paired homolog, Drosophila) antibody
Reactivity
Human
Target
ZIC5
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EIYGNPEVVRTIH
Field of Research
Neuroscience; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 1ug/ml, IHC-P: 3.75 μg/ml
Molecular Weight
68.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Small Intestine shows nuclear staining. Recommended concentration, 3-5ug/ml.WB: Preliminary experiments gave an approx 45kDa band in human brain lysates at 1 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 73kDda according to NP_149123. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
85416
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