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Goat anti-ZIC5 Antibody

Goat polyclonal antibody to ZIC5

Product Specifications

Product Name Alternative

Anti ZIC5 antibody, anti zinc finger protein of the cerebellum 5 antibody, anti zinc family member 5 protein antibody, anti Zic family member 5 (odd-paired homolog, Drosophila) antibody

Reactivity

Human

Target

ZIC5

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EIYGNPEVVRTIH

Field of Research

Neuroscience; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 1ug/ml, IHC-P: 3.75 μg/ml

Molecular Weight

68.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Small Intestine shows nuclear staining. Recommended concentration, 3-5ug/ml.WB: Preliminary experiments gave an approx 45kDa band in human brain lysates at 1 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 73kDda according to NP_149123. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

85416

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