Goat anti-Cystatin B / Stefin B Antibody
Goat polyclonal antibody to CSTB
Product Specifications
Product Name Alternative
Anti CSTB antibody, anti cystatin B (stefin B) antibody, anti PME antibody, anti CST6 antibody, anti STFB antibody, anti CPI-B antibody, anti stefin B antibody, anti liver thiol proteinase inhibitor antibody, anti EPM1 antibody, anti cystatin B antibody
Reactivity
Human
Target
Cystatin B / Stefin B
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QTNKAKHDELTYF
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1-0.3 μg/ml
Molecular Weight
11.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in human brain lysates at up to 1 μg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as small as the predicted size of approx. 11KDa according to NP_000091. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1476
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