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Goat anti-Cystatin B / Stefin B Antibody

Goat polyclonal antibody to CSTB

Product Specifications

Product Name Alternative

Anti CSTB antibody, anti cystatin B (stefin B) antibody, anti PME antibody, anti CST6 antibody, anti STFB antibody, anti CPI-B antibody, anti stefin B antibody, anti liver thiol proteinase inhibitor antibody, anti EPM1 antibody, anti cystatin B antibody

Reactivity

Human

Target

Cystatin B / Stefin B

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QTNKAKHDELTYF

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.1-0.3 μg/ml

Molecular Weight

11.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in human brain lysates at up to 1 μg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as small as the predicted size of approx. 11KDa according to NP_000091. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1476

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