GAPDH (C Terminus) Loading Control Antibody
Goat polyclonal antibody to GAPDH
Product Specifications
Product Name Alternative
Anti GAPDH antibody, anti glyceraldehyde-3-phosphate dehydrogenase antibody, anti HGNC:4141 antibody, anti G3PD antibody, anti GAPD antibody, anti MGC88685 antibody, anti OTTHUMP00000174431 antibody, anti OTTHUMP00000174432 antibody, anti aging-associated gene 9 protein antibody, anti glyceraldehyde 3-phosphate dehydrogenase antibody
Reactivity
Human, Rat
Target
GAPDH (C Terminus) Loading Control
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
HQVVSSDFNSDT
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.001-0.003 μg/ml, IHC-P: 2.5 μg/ml, IF/ICC: 10μg/ml
Molecular Weight
36.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037) . Recommended concentration: 0.01-0.03 μg/ml.
Prediction Reactivity
Canine, Mouse, Porcine
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
2597
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