Sheep Interferon Beta (IFNb) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Beta (IFNb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Beta (IFNb) in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
IFNB1, IFN-B, IFB, IFF, IFNB, Interferon Beta 1 Fibroblast, IFN Beta
Reactivity
Sheep
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
12.7 pg/mL
Notes
For research use only.
Applications Notes
Dynamic Range
31.25-2000 pg/mL
Available Sizes
Frequently Asked Questions
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