Zebrafish Gonadotropin Releasing Hormone (GnRH) ELISA Kit

GNRH1 ELISA Kit, GRH ELISA Kit, LNRH ELISA Kit, LHRH ELISA Kit, LH-RH I ELISA Kit, Luliberin ELISA Kit, Luteinizing-Hormone Releasing Hormone ELISA Kit, Progonadoliberin-1 ELISA Kit, Gonadoliberin I ELISA Kit, Gonadorelin ELISA Kit, GnRH-associated peptide 1 ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish GnRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.