Human Anti-double stranded DNA Antibody IgG (dsDNA) ELISA Kit

Siyuan Lai 1, Ning Li 2, Songyue Chen 2, Yanxin Wang 2, Dengxiao Hong 2, Wenyan Xu 2, Zhongxin Chen 2, Baiqing Li 3, Changhao Xie Activated CD4+ T cells upregulate PD-L1 expression on B cells through CD40/CD40L signaling and direct contact in systemic lupus erythematosus Clin Immunol ., (2025)

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human dsDNA IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dsDNA IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human dsDNA IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dsDNA IgG in the samples is then determined by comparing the OD of the samples to the standard curve