Mouse Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit

α-SMA, ACT-A2 ELISA Kit, ACTSA ELISA Kit, ACTVS ELISA Kit, Asma ELISA Kit, GIG46 ELISA Kit, Cell growth-inhibiting gene 46 protein ELISA Kit, Smooth Muscle Actin ELISA Kit

Standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACTa2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACTa2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACTa2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACTa2 in the samples is then determined by comparing the OD of the samples to the standard curve