Human Interferon Regulatory Factor 4 (IRF4) ELISA Kit

Interferon regulatory factor 4 ELISA Kit, IRF 4 ELISA Kit, IRF-4 ELISA Kit, Irf4 ELISA Kit, IRF4_HUMAN ELISA Kit, LSIRF ELISA Kit, Lymphocyte specific interferon regulatory factor ELISA Kit, Lymphocyte specific IRF ELISA Kit, Lymphocyte-specific interferon regulatory factor ELISA Kit, Multiple myeloma oncogene 1 ELISA Kit, MUM 1 ELISA Kit, MUM1 ELISA Kit, NF EM5 ELISA Kit, NF-EM5 ELISA Kit, NFEM5 ELISA Kit, PU.1 interaction partner ELISA Kit, Sfpi1/PU.1 interaction partner ELISA Kit, Transcriptional activator PIP ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IRF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IRF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IRF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IRF4 in the samples is then determined by comparing the OD of the samples to the standard curve