Human Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) ELISA Kit

ACS4 ELISA Kit, FACL4 ELISA Kit, LACS4 ELISA Kit, MRX63 ELISA Kit, MRX68 ELISA Kit, Fatty-Acid-Coenzyme A Ligase, Long-Chain 4 ELISA Kit, Mental Retardation, X-Linked 63 ELISA Kit, Lignoceroyl-CoA Synthase ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACSL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACSL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACSL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACSL4 in the samples is then determined by comparing the OD of the samples to the standard curve