Human Folic Acid (FA) ELISA Kit

VB9 ELISA Kit, Vitamin B9 ELISA Kit, Folacin ELISA Kit, Folate ELISA Kit, V ELISA Kit, Itamin M ELISA Kit, Vitamin M ELISA Kit, Vitamin Bc ELISA Kit, Pteroyl-L-Glutamic Acid ELISA Kit, Pteroyl-L-Glutamate ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human FA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FA in the samples is then determined by comparing the OD of the samples to the standard curve.