Chicken Apurinic/Apyrimidinic Endonuclease 1 (APEX1) ELISA Kit

APE ELISA Kit, APE1 ELISA Kit, APEN ELISA Kit, APEX ELISA Kit, APX ELISA Kit, HAP1 ELISA Kit, REF1 ELISA Kit, Multifunctional DNA Repair Enzyme ELISA Kit, APEX Nuclease 1 ELISA Kit, Redox factor-1 ELISA Kit, DNA- (apurinic or apyrimidinic site) lyase ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken APEX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken APEX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken APEX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken APEX1 in the samples is then determined by comparing the OD of the samples to the standard curve