Human Cytochrome P450 2A6 (CYP2A6) ELISA Kit

CYP2A6 ELISA Kit, Cytochrome P450 ELISA Kit, I ELISA Kit, Cytochrome P450 ELISA Kit, I ELISA Kit, Cytochrome P450 IIA3 ELISA Kit, CYPIIA6 ELISA Kit, Coumarin 7-hydroxylase ELISA Kit, CYP2A3 ELISA Kit

Standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP2A6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP2A6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP2A6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP2A6 in the samples is then determined by comparing the OD of the samples to the standard curve