Dog Kallistatin (KAL) ELISA Kit

SERPINA4 ELISA Kit, KLST ELISA Kit, KST ELISA Kit, PI4 ELISA Kit, Serpin Peptidase Inhibitor Clade A Member 4 ELISA Kit, Tissue Kallikrein Inhibitor ELISA Kit, Kallikrein Binding Protein ELISA Kit, Peptidase inhibitor 4 ELISA Kit

Standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog KAL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog KAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog KAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog KAL in the samples is then determined by comparing the OD of the samples to the standard curve