Human Phoenixin-20 (PNX-20) ELISA Kit

Small integral membrane protein ELISA Kit, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa ELISA Kit, MITRAC7 ELISA Kit, Phoenixin-14 ELISA Kit, PNX-14 ELISA Kit, Phoenixin-20 ELISA Kit, PNX-20 ELISA Kit, SMIM20 ELISA Kit, C4orf52 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNX-20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNX-20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNX-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNX-20 in the samples is then determined by comparing the OD of the samples to the standard curve