Human Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

WAF1 ELISA Kit, p21 ELISA Kit, CIP1 ELISA Kit, p21WAF1 ELISA Kit, CAP20 ELISA Kit, CDKN1 ELISA Kit, MDA-6 ELISA Kit, SDI1 ELISA Kit, p21CIP1 ELISA Kit, CDK-Interacting Protein 1 ELISA Kit, Melanoma differentiation-associated protein 6 ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDKN1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDKN1A in the samples is then determined by comparing the OD of the samples to the standard curve