Plant Indole 3 Acetic Acid (IAA) ELISA Kit

Sohail Mumtaz 1, Rida Javed 2, Juie Nahushkumar Rana 2, Madeeha Iqbal 2, Eun Ha Choi Pulsed high power microwave seeds priming modulates germination, growth, redox homeostasis, and hormonal shifts in barley for improved seedling growth: Unleashing the molecular dynamics Free Radic Biol Med, (2024)

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant IAA in the samples is then determined by comparing the OD of the samples to the standard curve.