Human B- and T-lymphocyte attenuator (BTLA) ELISA Kit

B and T lymphocyte associated protein ELISA Kit, B and T lymphocyte attenuator ELISA Kit, B and T lymphocyte associated ELISA Kit, B- and T-lymphocyte attenuator ELISA Kit, B- and T-lymphocyte-associated protein ELISA Kit, BTLA ELISA Kit, BTLA_HUMAN ELISA Kit, BTLA1 ELISA Kit, CD272 ELISA Kit, CD272 antigen ELISA Kit, FLJ16065 ELISA Kit, MGC129743 ELISA Kit

Standard: 30 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BTLA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BTLA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BTLA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BTLA in the samples is then determined by comparing the OD of the samples to the standard curve