Cattle Beta-Carotene-15, 15'-Monooxygenase 1 (bCMO1) ELISA Kit

BCO1 ELISA Kit, BCDO ELISA Kit, BCDO1 ELISA Kit, BCMO ELISA Kit, Beta-carotene dioxygenase 1 ELISA Kit, Beta-carotene oxygenase 1 ELISA Kit, Beta, beta-carotene 15,15'-monooxygenase ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle bCMO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle bCMO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle bCMO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle bCMO1 in the samples is then determined by comparing the OD of the samples to the standard curve