Pig Transforming Growth Factor Alpha (TGFa) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Alpha (TGFa) .
Product Specifications
Product Name Alternative
TGF Alpha, ETGF ELISA Kit, TGF-A ELISA Kit, Protransforming Growth Factor alpha ELISA Kit, EGF-Like TGF ELISA Kit
UniProt
Q06922
Reactivity
Porcine
Field of Research
Cancer Biology; Neuroscience; Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6 pg/mL
Notes
For research use only.
Applications Notes
Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TGFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TGFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TGFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TGFa in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL
Available Sizes
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