Pig Actin Beta (ACTb) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb) .
Product Specifications
Product Name Alternative
ACT-B ELISA Kit, PS1TP5BP1 ELISA Kit, Actin, cytoplasmic 1 ELISA Kit, Actin, cytoplasmic 1, N-terminally processed ELISA Kit
UniProt
Q6QAQ1
Reactivity
Porcine
Field of Research
Cancer Biology; Neuroscience; Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.56 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ACTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ACTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ACTb in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL
Available Sizes
Frequently Asked Questions
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