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Pig Integrin Beta 1 (ITGb1) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 1 (ITGb1) .

Product Specifications

Product Name Alternative

CD29 ELISA Kit, MSK12 ELISA Kit, ITG-B1 ELISA Kit, FNRB ELISA Kit, GPIIA ELISA Kit, MDF2 ELISA Kit, MSK12 ELISA Kit, VLAB ELISA Kit, Fibronectin Receptor Beta Polypeptide ELISA Kit, Antigen CD29 Includes MDF2 ELISA Kit, Glycoprotein IIa ELISA Kit, VLA-4 subunit beta ELISA Kit

UniProt

Q9GLP0

Reactivity

Porcine

Field of Research

Neuroscience; Immunology & Inflammation

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.37 ng/mL

Notes

For research use only.

Applications Notes

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ITGb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ITGb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.79-50 ng/mL

Available Sizes

Frequently Asked Questions

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