Rat Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit

SLC1A3 ELISA Kit, EA6 ELISA Kit, GLAST1 ELISA Kit, Sodium-dependent glutamate/aspartate transporter 1 ELISA Kit, Solute Carrier 1 Member 3, Glial High Affinity Glutamate Transporter ELISA Kit, GLutamate Aspartate Transporter ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EAAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EAAT1 in the samples is then determined by comparing the OD of the samples to the standard curve