Mouse Nuclear Factor Kappa B p65 (NFκB-p65) ELISA Kit

NFKB3 ELISA Kit, p65 ELISA Kit, REL-A ELISA Kit, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3 ELISA Kit, Transcription Factor P65 ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve