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Mouse NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) .

Product Specifications

Product Name Alternative

AVP ELISA Kit, AGTAVPRL ELISA Kit, AII ELISA Kit, AII/AVP ELISA Kit, C1orf7 ELISA Kit, CIAS1 ELISA Kit, CLR1.1 ELISA Kit, FCAS ELISA Kit, FCU ELISA Kit, MWS ELISA Kit, NALP3 ELISA Kit, PYPAF1 ELISA Kit, Cryopyrin ELISA Kit, Cold Induced Autoinflammatory Syndrome 1 ELISA Kit, Caterpiller-Like Receptor 1.1 ELISA Kit

UniProt

Q8R4B8

Reactivity

Mouse

Field of Research

Immunology & Inflammation; Cell Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.052 ng/mL

References & Citations

Hala A Ahmed 1, Ahmed A Shaaban 2, Mirhan N Makled 3, Tarek M Ibrahim G protein-coupled estrogen receptor selective agonist, G1, improves the molecular and biochemical markers in a cisplatin mouse model of CKD Chem Biol Interact ., (2024), Sara H Hazem 1, Karim M Saad 2, Mahmoud M Samaha Protective effects of BTK inhibition by acalabrutinib on cisplatin-induced renal and testicular injury in mice: Modulation of mTOR/AMPK, NLRP3/GSDMD-N, and apoptotic pathways Int Immunopharmacol ., (2025), Fahad O Alenezi 1, Manar A Nader 2, Dalia H El-Kashef 2, Marwa E Abdelmageed Dapansutrile mitigates concanavalin A- induced autoimmune hepatitis: Involvement of NLRP3/IL-1β and JNK/ p38 MAPK pathways Biomed Pharmacother, (2025), Hani M. Alrawili , Mahmoud Elshal , Marwa S. Serrya* and Dina S. El-Agamy Hepatoprotective Effect of Cynarin on Alpha-Naphthyl Isothiocyanate-Induced Cholestatic Liver Injury: Associated Modulation of TXNIP/NLRP3 and HMGB1/NF-κB Signaling Cascades Pharmaceuticals, 19 (2), 280 (2026)

Pubmed id

38795875, 39938312, 40164046

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NLRP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NLRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NLRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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