Mouse Atrial Natriuretic Peptide (ANP) ELISA Kit

ANH ELISA Kit, ANF ELISA Kit, CDD ELISA Kit, Atrial Natriuretic Factor ELISA Kit, Atrial Natriuretic Hormone ELISA Kit, Atriopeptin ELISA Kit, Cardionatrine ELISA Kit, Cardiodilatin ELISA Kit

Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ANP in the samples is then determined by comparing the OD of the samples to the standard curve