General Dipalmitin (PP) ELISA Kit

1,3-Dipalmitin ELISA Kit, 1,3-Di- (Hexadecanoyl) -Glycerol ELISA Kit, 1,3-Dipalmitoylglycerol ELISA Kit, 1,3-Dipalmitoyl-Glycerol, Glyceryl 1,3-Dipalmitate ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PP in the samples is then determined by comparing the OD of the samples to the standard curve.