Rat Serum Amyloid A (SAA) ELISA Kit

SA-A ELISA Kit, SAA1 ELISA Kit, PIG4 ELISA Kit, TP53I4 ELISA Kit, Amyloid protein A ELISA Kit, Amyloid fibril protein AA ELISA Kit, Serum amyloid A-1 protein ELISA Kit

Standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SAA in the samples is then determined by comparing the OD of the samples to the standard curve