Human Somatostatin (SST) ELISA Kit

GHIH ELISA Kit, SRIF ELISA Kit, SMST ELISA Kit, GHRIH ELISA Kit, GHRIF ELISA Kit, Growth Hormone Releasing Inhibiting Hormone ELISA Kit, Somatotropin Release-Inhibiting Factor ELISA Kit, Somatostatin-14 ELISA Kit, Somatostatin-28 ELISA Kit

Standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SST in the samples is then determined by comparing the OD of the samples to the standard curve