General Quinolinic Acid (QA) ELISA Kit

J Lewkowicz, A Tankiewicz-Kwedlo, D Pawlak, M Kiluk, K Lagoda, I Kowalska Kynurenines in Heart Failure With Preserved Ejection Fraction: An Influence of Type 2 Diabetes researchsquare.com, (2024)

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with QA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to QA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of QA in the samples is then determined by comparing the OD of the samples to the standard curve.