Rat Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) ELISA Kit

Kir4.1 ELISA Kit, Kir1.2 ELISA Kit, ATP-sensitive inward rectifier potassium channel 10 ELISA Kit, ATP-dependent inwardly rectifying potassium channel Kir4.1 ELISA Kit, Inward rectifier K (+) channel Kir1.2 ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KCNJ10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KCNJ10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KCNJ10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KCNJ10 in the samples is then determined by comparing the OD of the samples to the standard curve