SR FLICA Caspase 8 Assay Kit

1. Dilute 10X Cellular Assay Buffer 1:10 with diH2O. 2. Prepare samples and controls in fresh cell culture medium or 1X Cellular Assay Buffer. 3. Reconstitute R110- (RR) 2 with 50 μL DMSO. 4. Dilute R110- (RR) 2 1:5 by adding 200 μL PBS. 5. Add diluted R110- (RR) 2 to each sample at 1:50 (e.g. add 10 μL of diluted R110- (RR) 2 to 490 μL of sample) . 6. Incubate while protected from light. 7. If desired, label with additional stains, such as Hoechst 33342, DAPI, or an antibody. 8. Analyze with a flow cytometer or fluorescence microscope. R110- (RR) 2 excites at 500 nm and emits at 525 nm.