FAM-FLICA Caspase 8 Assay Kit

1. Prepare samples. 2. Create controls with CCCP. 3. Dilute 10X Assay Buffer 1:10 with diH2O. 4. Reconstitute MitoPT JC-1 with DMSO. 5. Dilute MitoPT JC-1 with 1X Assay Buffer. 6. Add MitoPT JC-1 to each sample. 7. Incubate 15-30 minutes. 8. Wash cells: add 1X Assay Buffer and spin cells (twice) . 9. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.