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UBE2F/NEDD8-conjugating enzyme Antibody

Goat polyclonal antibody to UBE2F

Product Specifications

Product Name Alternative

Anti UBE2F antibody, anti ubiquitin-conjugating enzyme E2F (putative) antibody, anti MGC18120 antibody, anti NCE2 antibody, anti NEDD8 conjugating enzyme antibody, anti NEDD8-conjugating enzyme antibody

Target

UBE2F/NEDD8-conjugating enzyme

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RNKVDDYIKRYAR

Field of Research

Protein Biochemistry

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1 μg/ml

Molecular Weight

21.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Prediction Reactivity

Bovine, Canine, Human, Mouse, Porcine, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

140739

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