UBE2F/NEDD8-conjugating enzyme Antibody
Goat polyclonal antibody to UBE2F
Product Specifications
Product Name Alternative
Anti UBE2F antibody, anti ubiquitin-conjugating enzyme E2F (putative) antibody, anti MGC18120 antibody, anti NCE2 antibody, anti NEDD8 conjugating enzyme antibody, anti NEDD8-conjugating enzyme antibody
Target
UBE2F/NEDD8-conjugating enzyme
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RNKVDDYIKRYAR
Field of Research
Protein Biochemistry
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1 μg/ml
Molecular Weight
21.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
Prediction Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
140739
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