Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Colorimetric Assay Kit
In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm. The absorbance increase rate reflect AKP activity.
Product Specifications
Field of Research
Signal Transduction
Storage Conditions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Notes
For research use only.
Applications Notes
In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm; the absorbance increase rate reflect AKP activity.
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