Human Phosphatidylinositol-glycan biosynthesis Class F Protein (PIGF) ELISA Kit

PHOSPHATIDYLINOSITOL GLYCAN ELISA Kit, Phosphatidylinositol glycan biosynthesis class F protein ELISA Kit, Phosphatidylinositol-glycan biosynthesis class F protein ELISA Kit, PIG F ELISA Kit, PIG-F ELISA Kit, PIGF ELISA Kit, PIGF_HUMAN ELISA Kit, PIGF1 ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.