Cattle Antibacterial Protein LL-37 (LL-37) ELISA Kit

CAP18 ELISA Kit, FALL39 ELISA Kit, HSD26 ELISA Kit, LL37 ELISA Kit, 18 kDa Cationic Antimicrobial Protein ELISA Kit, Antibacterial protein FALL-39 ELISA Kit, FALL-39 peptide antibiotic ELISA Kit

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve